🧬 BioFlo Chromatography Column Sizer

Calculate preparative chromatography bed volumes ($CV = \pi r^2 L$), linear flow velocities ($u = \frac{Q}{A}$ cm/h), residence times ($\tau = \frac{CV}{Q}$), and column packing quality (HETP & peak asymmetry $A_s$).

Column Geometry & Bed Height

Column Inner Diameter D (cm)

Packed Bed Height L (cm)

Resin Packing Compression Factor Settled slurry volume = Bed Volume × Compression

Linear Flow Velocity (cm/h)

Column Packing Evaluation (Pulse Test)

Retention Volume / Time (tR)

Peak Width at Half Height W_h (min or mL)

Front Width at 10% Height (a)

Rear Width at 10% Height (b)

Calculated Bioprocess Column Parameters

392.7 mL

Column Volume (CV)

49.1 mL/min

Volumetric Flow Rate (Q)

8.0 min

Residence Time (τ)

451.6 mL

Settled Resin Required

967 plates

Theoretical Plates (N)

0.021 cm

HETP Plate Height

1.22

Peak Asymmetry (As)

✓ Accepted

Packing Quality (0.8 - 1.4)