🌱 BioFlo OD-to-Cell Density Calculator

Convert spectrophotometric optical density measurements (OD600) into Dry Cell Weight ($DCW$ g/L), viable cell concentration (cells/mL), and wet cell mass.

Spectrophotometer Input
Samples with OD > 0.8 must be diluted to maintain Beer-Lambert linearity.
Calculated Biomass & Density
4.80
Corrected Broth OD600
1.73 g/L
Dry Cell Weight (DCW)
3.84 × 10⁹
Estimated Cells / mL
7.79 g/L
Wet Cell Weight (~4.5× DCW)