This article details the advanced computational modeling required for biocatalytic cascades, integrating complex reaction kinetics (like Michaelis-Menten) with mass transfer phenomena (axial dispersion and diffusion) to accurately predict system performance and operational stability.
The purification of bioproducts from complex biological feedstocks requires highly efficient, integrated separation trains. This article details how combining membrane filtration (size-based separation) and adsorption chromatography (affinity-based separation) achieves high purity and yield in biomanufacturing.
This article explores the fundamental concepts of pH, defining what it is and how it is measured. We will delve into the chemistry of acids and bases, examining their properties, reactions, and the critical role…
Novel chromatographic resins are revolutionizing bioseparation by offering multi-modal interactions and folding assistance, ensuring high purity while maintaining the native, active structure of therapeutic proteins.
This article explores the application of advanced Computational Fluid Dynamics (CFD) models to optimize bioreactor performance. Key areas include predicting shear stress distribution, optimizing sparging strategies, and maintaining optimal dissolved oxygen levels for enhanced bulk…
Downstream processing (DSP) is the critical, complex, and often rate-limiting step in viral vector manufacturing. Optimization involves maximizing the selectivity and efficiency of physical separation mechanisms, utilizing advanced chromatography and filtration techniques to achieve high…
In situ product removal (ISPR) strategies are critical engineering solutions designed to continuously reduce the concentration of target bioproducts or inhibitory metabolites from the bioreactor broth, enabling the successful cultivation of high-titer bioproducts.
Scaling up bioprocesses requires overcoming critical limitations related to mass transfer, shear stress, and waste accumulation. Advanced bioreactor designs, including perfusion systems and packed-bed reactors, are essential for maintaining high cell viability and productivity in…
This article details the modification of standard cell population dynamics models to incorporate a shear-damage term, providing a more accurate representation of biological systems subjected to fluid forces.
This article explores the various methods of cryopreservation, detailing the use of liquid nitrogen and other cryoprotectants to preserve biological materials.